qEV Exosome Isolation

Rapid, High Precision Exosome Isolation.

qEV provides the most rapid and effective EV/exsosome isolation system available. qEV is based on Size Exclusion Chromatography (SEC), and takes around 15 minutes to get a pure sample of intact exosomes, significantly purer than is possible with concentration methods such as ultracentrifugation (UC) or precipitation reagent kits. The resulting samples are consistent, standardisable and repeatable, which is required for both research and clinical testing. qEV is now the gold standard for exosome isolation.

Rapid, Simple & Reliable

With qEV it takes around 15 minutes to get a pure sample of intact exosomes. Reliability has been proven in a large scale inter-laboratory trial.

Standardisable & Reproducible

Each column is stardardised, quality assured and certified to ISO 13485 standard (Medical Devices) making it perfect for clinical testing.

Pure, Clean Isolation Samples

qEV columns provide clean and pure samples without affecting the structure and function of the exosomes.

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Rapid. Gentle. Precise.

qEV offers fast, gentle & precise isolation exosomes, removing more than 99% of soluble proteins. This makes qEV-isolated EVs/exosomes suitable for electron microscopy, proteomics, RNA analysis, and physical measurement.

ISO 13485 Certified
Quality certified to ISO 13485: 2016 - Medical Devices
Pure
Removes > 99% of soluble proteins
Optimised For Your Research
The rapidly growing qEV range now includes 8 different columns.

The new standard in exosome isolation.

Prior to the advent of SEC columns, UC was regarded as the gold standard for EV concentration and isolation. UC is time consuming and cannot provide a consistent standardised sample. The very high forces in UC, up to 200,000g, affect, disrupt and aggregate the vesicles, which may in turn invalidate much of the research. Moreover, aggregated proteins and nucleic acid contaminants are present in the pellet containing the EVs. Density gradient centrifugation (DGC) aims to improve the purity of UC derived exosomes but increases the complexity and time of the procedure even further. Standardised sampling across different laboratories is not achievable with either UC or DGC.

Precipitation reagent kits have been adapted for exosome/EV purification. They are typically PEG based and sediment a wide range of product, not just EVs. Published independent data indicates these fractions are heavily contaminated with non-EV material because these reagents cause co-precipitation of proteins, lipoproteins and other biological components. qEV SEC columns provide clean samples without affecting the structure or function of the EV. Precipitation reagent kits provide dirty samples that vary from batch to batch. The leading researchers in the EV field no longer use or recommend precipitation products.

The new standard in extracellular vesicle isolation.

Prior to the advent of SEC columns, UC was regarded as the gold standard for EV/exosome concentration and isolation. UC is time consuming and cannot provide a consistent standardised sample. The very high forces in UC, up to 200,000g, affect and disrupt the vesicles, which may in turn invalidate much of the research. Moreover, aggregated proteins and nucleic acid contaminants are present in the pellet containing the EVs. Density gradient centrifugation (DGC) aims to improve the purity of UC derived exosomes but increases the complexity and time of the procedure even further. Standardised sampling across different laboratories is not achievable with either UC or DGC.

Precipitation reagent kits were adapted for exosome/EV use fromvirus preparation. They are typically PEG based and supposedly sediment the exosomes only. Published independent data indicates these fractions are heavily contaminated with non-EV material because these reagents cause co-precipitation of proteins, lipoproteins and other biological components. The leading researchers in the EV field no longer use or recommend these products. qEV SEC columns provide clean samples without affecting the structure or function of the EV. Reagent kits provide dirty samples that vary from batch to batch and are no longer recommended for exosome purification.

How qEV Isolation Works

qEV is based on size exclusion chromatography (SEC). Size exclusion chromatography uses a stationary phase consisting of porous resin particles. Molecules smaller than the isolation range (35nm+ or 70nm+) are slowed because they enter into the pores of the stationary phase. Larger particles which cannot enter the pores flow around the resin and are eluted from the column earlier. Molecules and small particles that enter the pores have longer retention times and elute later.

qEV - Now Automated & Highly Scalable

The world’s first automated, smart EV isolation system is here. A significant enhancement to the qEV platform, the Izon Automatic Fraction Collector (AFC) allows for fast, precise, automated isolation of EVs. Until now the qEV process has been labour intensive and difficult to scale. The AFC automates the qEV isolation process, eliminating human error, enabling high-precision large-scale, standardisable exosome isolation. A new era of EV isolation is here.

Learn More

How qEV Works

qEV is based on size exclusion chromatography (SEC). Size exclusion chromatography uses a stationary phase consisting of porous resin particles. Molecules smaller than the isolation range (35nm+ or 70nm+) are slowed because they enter into the pores of the stationary phase. Larger particles which cannot enter the pores flow around the resin and are eluted from the column earlier. Molecules and small particles that enter the pores have longer retention times and elute later.

qEV - Now Automated

The world’s first automated, smart exosome isolation system is here. A significant enhancement to the qEV platform, the Izon Automatic Fraction Collector (AFC) allows for fast, precise, automated isolation of EVs. Until now the qEV process has been labour intensive and difficult to scale. The AFC automates the qEV isolation process, eliminating human error, enabling high-precision large-scale, standardisable exosome isolation. A new era of exosome isolation is here.

Learn More

Choose an Exosome Isolation Column Optimised For Your Research.

There are currently 4 different Izon columns sizes to choose from, each available in 2 isolation ranges (designated 35 or 70). As each column has its own advantages, we’ll guide you through the process of choosing the right one for your research. The important selection considerations are sample volume and expected particle sizes. All 8 of our columns benefit from our ISO 13485:2016 certification.

Step 1 - Choose Your Column Size

qEVsingle

qEVoriginal

qEV2

qEV10

qEV100

< 150 µL
Sample loading (recommended for highest purity).
Ideal for clinical samples, RT-PCR
Optimised for small samples. No RNA carryover (single use). Compatible with the Automatic Fraction Collector.
Single Use
Cost efficient.

< 500 µL
Sample loading (recommended for highest purity).
Ideal for higher volume research
The original and most popular qEV column. Compatible with the Automatic Fraction Collector.
Reusable
Up to 5 times.

< 2 ml
Sample loading (recommended for highest purity).
Ideal for larger clinical samples & RNA preparation
Includes double Leur Lock fitting. Compatible with the Automatic Fraction Collector.
Reusable
Up to 5 times.

< 10 ml
Sample loading (recommended for highest purity).
Ideal for large volume cell culture supernatant
Includes double Leur Lock fitting. Compatible with the Automatic Fraction Collector.
Reusable
Up to 5 times.

< 100 ml
Sample loading (recommended for highest purity).
Ideal for large scale, fast EV isolation
Includes double Leur Lock fitting. Currently not compatible with the Automatic Fraction Collector.
Reusable
Up to 5 times.